However, the efficiency of transfection of variant cells is unreliable and ranges from susceptible to resistant.
Short hairpin RNA-mediated stable knockdown of MMP-1 inhibited the invasive ability of MDA-MB 231 variant cells in vitro, and inhibited breast cancer growth when the cells were injected into the mammary fat pad of nude mice.
Okadaic acid, a phosphatase inhibitor, increased S6P and MBP kinase activities in both wild-type and variant cells.
Luciferase-expressing 4T1 variant cell pools and lines with increased propensity for metastasis to brain, liver, and bone have recently been isolated (to be published elsewhere).
Both non-variant and LCS6 variant cells formed endometriosis-like lesions with both glandular and stromal components (Fig 5a).
Among several differentiating agents, only dcAMP, which alone induced attachment and some process formation, increased laminin-mediated attachment and process formation of two SCLC cell lines, NCI-N417 a variant cell line, and NCI-H345, a classic cell line.
The DNA expression-construct pCMV-HA-CA-MEK5 [ 41] was used to derive MEK5 over-expressing variants of MCF-7 (N variant) cell line and selected with G418 (Gibco, Paisley, UK).
Although the variant cells had reduced levels of protein kinase C as compared with the wild-type cells, the amount of membrane-bound enzyme increased in response to phorbol 12-myristate 13-acetate in both wild-type and variant cells.
Two variant cell lines, TA3/Ha and TA3/St, differing in cell coat and surface charge, did not differ in the extent of infiltration.
Parental cells and variant cell lines were cultured in high-glucose (4.5 g/l) DMEM medium (Lonza, Basel, Switzerland) supplemented with 10% FCS, non-essential amino acids (both Life Technologies, Carlsbad, CA, USA) and antibiotics at 37 °C and 5% CO2.
