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The histamine release induced by the Bos d 5 B mutant H146P, that was shown by the MS analysis to exist mainly as a monomer, show decreased histamine release capacity when compared to the recombinant Bos d 5 (B variant) and native Bos d 5 (AB variants) (Figure 3B).
The alteration of secondary protein structure and functional domain sites between the variant and native forms due to the presence of variable number of TFFDMS repeat units encoding the amino acid residues in the functional domains of four seed and pod trait-associated TF genes was observed (Supplementary Fig. S6B).
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In the EMSAs with and without the addition of NEM, the effects of the Cys-to-Ser mutation were more pronounced than the difference we observed between the native Cys variant and the native Ser variant (Figure 7A, C).
All encoded CipA variants (synthetic and native) were fused at the carboxy-terminal with the Flag epitope tag, allowing the detection and quantification of the secreted scaffoldin proteins by western blot analysis.
At pH 4.0 and 5.0, the S168W and S168W-environment variants and the native C. subvermispora MnP6 showed 90 to 100% residual activities after both 4 and 24 h of incubation, while VP1 showed about 80% activity.
They concluded that the repertoires responding to the peptide variants and the native tumor antigen were subtly different but over-lapping, and that the observed functional differences were due to subtle structural changes in the TCR.
The optimal pH values for substrate oxidation by the two C. subvermispora MnP6 variants and the native P. ostreatus VP1 were determined by measuring the oxidation of saturating concentrations of RB5 (15 μM) and VA (6 mM) in 0.1 M BR buffer of pH 1.6 to 5.0, as described above.
This array was designed for use in Latinos and involved preferential selection of variants in European and Native American ancestries (Hoffmann et al. 2011b), which might explain the lower coverage in the AFR group.
Primers for β-actin were from Shorter et al. (14); those for all splice variants of FP and native FP (altFPs) were from Liang et al. (30); those for altFP2 5 were from U.S. patent 7320871 (SEQ ID no. 37 and 38); and those for FP and altFP1 were designed from the human FP gene (GenBank accession no. NC_000001.10) using the Ensembl Genome Browser (http://www.ensembl.org).org
Recently, its prevalence was increased in west and west-central Africa, where this viral variant is native and predominantly transmitted within heterosexual population, also in male at-risk populations, such as continental men who have sex with men (MSM) [ 10].
In particular, rs7754840 may better capture the causal variant in the Japanese American and Native Hawaiian populations, which is not implausible given their recent shared ancestry.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com