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Differentially expressed genes were defined as genes with at least 2 fold variance of expression levels in M1, M2 and M IL-23) polarized M IL-23ages compolarizedmacrophagesges.
The number of slides might be determined from the magnitude and variance of expression change, false-positive rate, and desired power.
A robust version of the formula for updating the variance of expression distribution of targets has also been investigated.
We measured the expression variability by calculating the mean and variance of expression changes of each gene in the yeast genome in response to each form of perturbation.
These data indicated that the variance of expression of ribosomal protein genes caused by replication within one tissue (both of biological and technical origin) was much smaller than the variation between tissues.
GeneChIP.CEL files were loaded into DNA-Chip Analyzer (dCHIP) [79] (http://www.dchip.org) for normalization to reduce technical variation between chips, standardization to reduce variance of expression level estimates by accounting for probe differences, and analysis using model-based expression indexes (MBEI).
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Variances of expression profiles of genes in the diagonal of C are normalized so that their values reflect their quantile in the whole distribution of variances [9] (i.e. these values range from 0 to 1).
For statistics, and for Welch statistics, ; where and are the variances of expression levels for probe set i in the patient and control groups.
The analysis of expression levels of human islet tissue for T2D showed that the variances of expression levels of the genes were caused by the aberrant DNA methylation and the intensities of chromatin modifications.
The proportionality of the 2 variances was again confirmed, while another noteworthy finding was that in the case of heterozygotes, the robustness was further enhanced (suppresses the variances of expression).
We measured gene expression variation by the logarithm of the variance of the expression levels of each gene across the 40 Ca and Na exposure conditions [ 19].
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