These scale differences are resulted from the fact that the feature variance in Figure 3 is computed globally from the entire test features while the averaged variance model is represented as the average value of the individual variance models, each of which covers its small acoustic model space.
We illustrate how BitSeq handles biological replicates to account for this variance in Figure 5, by showing the modelling process for one example transcript given only two biological replicates for each of two conditions.
The difference between the magnitudes of the PE variances in Figures 2 (linear) and 3 (cubic) illustrate both that the variance contributed by PE effects, particularly of males, increases with age, but that the effects are less extreme than a linear regression would indicate.
end{aligned} We plot the conditional variances in Figure 2.
To illustrate the accuracy of the estimation, we also depicted the RMSE of estimated K with different setting of V and variances in Figure 3.
So in order to improve the simulations, that is in order to remove this growth trend in the simulated variances in Figure 5, it would be really worthwhile to restrict the parameters estimates of the model to those belonging to this subset.
However without providing measures of variance in these Figures, it remains unclear whether there was no difference between treatments.
For the specific case of s 1, 0 = 1 2, the total contribution for which the maximal variance occurs in Figure 3, we illustrate the variance at several locations in the allowed range for s 1, 0 f and s 1, 0 m.
The variance shown in Figure 4 suggests potential issues either with variability in the underlying hardware, contention on the same physical system, or variability through the hypervisor.
I found that variation in the rate of visits explains nearly all the total observed variance in V (Figure 5).
