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These WGDs in connection with different retention and pseudogenization rates might explain the variance in coverage within this clade.
Because of the high variance in coverage across the hypervariable region, we did not include this region in our final assemblies or analyses.
Second, the genomic assemblies produced from Illumina sequencing of whole-genome-amplified haploid embryos do indeed have a substantially bigger variance in coverage across the genome.
In addition, variance in coverage is of importance to de-novo assembly pipelines and read mapping strategies, since local regions of unusually high coverage may be interpreted as duplications or masked as repeat elements [ 5, 6].
It has been shown that the Knapp-Hartung method outperforms inverse variance in coverage for the summary effect and that it is insensitive to the estimator of the heterogeneity used [ 20, 21].
Furthermore, the matched normal also allows WaveCNV to correct for anomalies present in the reference sequence including systematic variance in coverage, high repeat regions, unsequencable regions with consistently missing coverage and so forth.
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Our strategy is based on the multi-mapper-resolution (MMR) approach [Kahles and Rätsch personal communication: For each read with multiple possible mapping location MMR decides for the location, such that the variance in read coverage is minimized.] that decides on the read coverage distribution how to optimally choose one of several ambiguous alignments.
A weak but non-significant correlation was observed between mtGenome coverage and sequence distance from the rCRS, with a mere 1-31-3% the variance in mtGenome coverage attributed to sequence distance (data not shown).
Considerable variance in district coverage is evident: If Bawku East district is considered as a reference district, the relative odds of NHIS coverage in Bawku West is 50percentt lower, Bongo 30percentt lower, Garu-Tempane 51percentt lower and Talensi-Nabdan 37percentt lower while the relative odds of insurance coverage in Builsa was 28percentt higher and Bolgatanga 31percentt higher (Table 2).
The level of bargaining accounts for 70percentt of the cross-national variance in bargaining coverage in a sample of 48 countries (Visser et al. 2015).
In other words, 56% of the variance in mean coverage is accounted for by the value of ANC 4+.
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