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From the variable sites, 38 positions were found as singletons while the remaining 244 variable sites were parsimony informative.
Thirteen of the variable sites were parsimony-informative.
from ranks selected at random from the variable sites.
Five sites (of 47 variable sites) were informative in ITS1, 5 (of 26 variable sites) in ITS2, and no informative site was recorded in the 5.8S gene which contained 5 variable sites (Table 2).
These primers contain nucleotides corresponding to 11 and 19 variable sites, respectively (Table 3).
Out of 1092 variable sites, 270 (24%) had mutated more than once.
Forty-two of the 73 variable sites from the matrix were phylogenetically informative.
Variable sites can be differentiated by the representation of each AA at the site.
The alignment can also be used to extract variable sites for more traditional phylogenetic analyses.
All variable sites from the sequence alignment were used in MP analysis.
The number of variable sites was analyzed by the Sequence Data Explorer tool.
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