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Maximum methylation entropy may be observed in half-methylated regions with highly variable methylation patterns.
Highly variable methylation patterns were frequently observed at intermediate methylated loci.
However, the distribution of variances has a broad tail and many sites showed extremely variable methylation patterns.
Legend: Constant methylation patterns are correlated to loss of mRNA expression, while variable methylation patterns are not.
This suggests that variable methylation patterns could be a by-product of tumor dedifferentiation and possibly DNMT-1 (DNA methyltransferase 1) overexpression without regulatory links to mRNA-expression.
S100A2 showed more variable methylation patterns, with methylation ranging from partial methylation at one site (DAOY) to complete methylation at all sites (D283 Med).
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Primary EC tumor tissues showed a variable methylation pattern of the L1CAM promoter.
The priorly investigated genes, SERPINB5 (constant methylation pattern) and AKAP12 (variable methylation pattern) were included for this analysis.
The variable methylation pattern observed at the INS TSS suggests that methylation at this TSS could regulate tissue-specific expression in the tammar as it does in eutherians [ 78].
These analyses identified additional differences between the cell lines and the normal population and allowed us to better relate the significance of these chromosomal changes to variable DNA methylation patterns, as described below.
It is also possible that tissue-specific differences result in the variable DNA methylation patterns we observed in whole embryos.
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