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For XRD at low angle section, it was between 1° and 30° (2θ) at a step size of 0.0167° with variable divergence slit and 0.125° anti-scatter slit.
X-ray diffraction (XRD) patterns of the film formed on the metal surface with and without TPD addition were analysed using a PANalytical X'Pert Pro powder diffractometer in θ θ configuration with an X'Celerator detector and variable divergence and fixed receiving slits with Fe-filtered Co-Kα radiation (λ = 1.789 Å).
This is likely due to variable divergence across the family and the short sequence length [ 35].
Although the complete sequencing of mitochondrial DNA highlighted a sequence identity of 96%, variable divergence times have been proposed, ranging from 6 to 40 Mya [ 7, 10].
The three models include M0, speciation with no gene flow; M1, variable divergence times across the genome between sister species, which is interpreted as evidence for gene flow; and M2, the SIM3s model (Yang 2010; Zhu and Yang 2012), which includes an explicit migration parameter.
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Another important factor that can cause variable species divergence times over the genome is natural selection.
We sequentially analyzed and removed every known variable affecting divergence rates (Table 3), starting with sex chromosomes.
In this paper, I implement a likelihood ratio test of variable species divergence times over the genome, which may be considered a test of the null model of allopatric speciation without gene flow against the alternative model of parapatric speciation with gene flow.
In the article by M. S. O. Brieuc, C. D. Waters, J. E. Seeb, and K. A. Naish, (G3: Genes|Genomes|Genetics 4: 447–460) entitled "A Dense Linkage Map for Chinook salmon (Oncorhynchus tshawytscha) Reveals Variable Chromosomal Divergence After an Ancestral Whole Genome Duplication Event," Table 4 has been corrected.
In fact, the ascidian mitogenomes have already proved to be hypervariable in many genomic features, because they show very high sequence divergence, variable tRNA gene content, variable position of the longest noncoding region (NCR), and rampant gene order rearrangements even in congeneric and cryptic species (Iannelli, Pesole, et al. 2007; Gissi et al. 2008, 2010).
The likelihood ratio test (LRT) used in the triplet method requires many loci to achieve statistical power, because the historical signature of gene flow is recorded as variable gene tree divergence times, and differences in divergence times might be subtle if speciation was recent or if gene flow only occurred for a short time interval following speciation.
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