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Experiments with variable detector positions were performed and spectral line widths of ∼2 ns were achieved at the end of a 45 cm long linear oTOF MS with ∼3.7 keV energy ions.
Corrections were applied to account for variable detector efficiencies and dead time, photon attenuation and scatter and radioactive decay using the software provided by the manufacturer.
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Detector used was UV VIS variable wavelength detector.
HPLC was carried out on a system consisting of two pumps, an injector and a variable UV/Vis detector, and a sodium iodide crystal detector.
Reversed-phase high-performance liquid chromatography (RP-HPLC) analysis was performed with an UltiMate 3000 RS UHPLC pump, UltiMate 3000 RS Column Compartment (column oven temperature was set at 25 °C), UltiMate 3000 Variable Wavelength Detector (Dionex, Germering, Germany) and a radio detector (GabiStar, Raytest; Straubenhardt, Germany).
HPLC analyses were performed on a Pharmacia Biotech Äkta Basic (pump type P-900, variable wavelength detector) or a JASCO HPLC (pump type PU-2080 plus, variable wavelength detector).
A high performance liquid chromatography (HPLC) system was equipped with variable wavelength detector (VWD, SHMADZU, Japan).
Extracted samples were analysed immediately on an Agilent Technologies 1200 Infinity HPLC instrument with a gradient pump, an autosampler, a variable wavelength detector and ODS Hypersil column (250 × 4.6 mm2; 5 μm particle size).
The chromatographic system (Dalian, China) consisted of ultimate 3000 autosampler, ultimate 3000 pump, and ultimate 3000 variable wavelength detector.
Andrographolide content in the samples was detected and quantified by HPLC system, supplied by Agilent Technologies comprising 1100/1200 Column Thermostat, 1200 Variable Wavelength Detector, 1100/1200 Quaternary Pump and 100/1200 Thermostatted Autosampler.
Analyses were primarily performed on an Agilent 1200 HPLC system (Agilent Technologies, CA, USA), equipped with a variable wavelength detector (VWD).
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