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As the target value of the variable amplification the maximum amplitude is set to 90% of the total scale.
First, expression values were normalised to correct for variable amplification efficiencies across genes, as previouslyreported [ 28].
Two microsatellite markers (Lhoc10 and Lhoc26) showed variable amplification success across sampled ESUs and were therefore removed from the analysis.
However, variable amplification of transcripts may account for the disparity between the qRT-PCR and microarray results for IL6.
Variable amplification was likely due to localized sequence polymorphism within the primer binding site (i.e. small indels) and not the lack of microsynteny.
The efficiency for the amplification of each product was determined by serial dilutions of template cDNA and used to correct Ct values for variable amplification efficiencies.
Similar(46)
The total signal amplification is calculated from a fixed basic amplification and a variable gain component.
For example, microsatellites are often more variable, requiring amplification of fewer loci to achieve a high level of insight, whereas any given SNP can only have a maximum of four states.
In the case of the rDNA loci in the Triticeae, a further variable includes amplification events that increase the number of tandem copies of the genes at a given locus (Dubcovsky and Dvorak 1995).
The response variables for polymorphism, Polymorphism and observed heterozygosity were tested with the same explanatory variables as amplification success with the following deviation: the explanatory variable 'ΔTmH DNA -DNA hybridisation value' was dropped for the analysis of Polymorphism since we tested for polymorphism over a range of species.
Two major variables: the amplification process will change the abundance of the transcripts and low signal strength may results from inefficient probes.
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