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The Y-intercept provides with an estimate of the variability of the method.
In particular, the baseline sensitivity and variability of the method need to be assessed.
To estimate the variability of the method eight independent purifications were carried out (n = 8).
We can use these repeated data to measure the variability of the method.
Both the DNA isolation step and the PCR step contribute to the total variability of the method results.
Eight variable τ2 time delays were used ranging from 635 to 9,035 ms. The intraday variability of the method is 6.5% and interday variation 8.0%.
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The study design did not allow for determination of the intra- and inter-observer variability of the methods; however, the variability between the three specimens from each specimen was determined to be good (R = 0.684).
Undertaking reviews in this area is difficult because of the complexity inherent in the interventions, the variability of the methods used, and the difficulty of generalising findings across healthcare settings.
Moreover, the score intervals are very narrow, with a difference of only a few bacteria, and the real number of Lactobacillus morphotypes recognized may be influenced by the variability of the methods.
As by nature the variability of the sampling method and the reference method are included in the variability obtained for the NIR method during the validation phase, a real-time process monitoring exercise was performed to prove its fit for purpose.
The variability of this method, although not big, may be avoided in the future by most precise ones.
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