Exact(3)
In this study spatial variability of reference evapotranspiration over Greece is investigated using geostatistics.
Based on the unequivocal impact of reference gene selection on experimental outcome presented here and elsewhere [14], there is a clear need for guidelines concerning tolerable variability of reference genes used for data normalization [32].
Variability of reference gene amplifications ranged between 0.1 Cq (caprine YWHAZ, corresponding to 33 copies) and 0.4 Cq (bovine ACTB, corresponding to 5.1 10 copies).
Similar(57)
The BE limits would change as a function of within-subject variability of the reference product (reference-scaled average BE).
The guidance on warfarin recommended a four-way, fully replicated crossover design in order to scale BE limits to the variability of the reference product and compare test and reference product within-subject variability [ 22].
Utilization of multiple reference genes is a way to relieve the effect of expression variability of single reference genes (Vandesompele et al. 2002).
Cross-validation indicates that vigiRank is not over-fitted to sampling variability of the reference dataset: the mean cross-validated AUC is marginally below that for the reference dataset as a whole, the selected predictors and their coefficients are remarkably consistent over the five cross-validation iterations, and vigiRank consistently outperforms IC025 over all iterations.
The similarity-based methods as the modern analog technique are, in principle (i.e. when the analogs have a true physical sense), able to reproduce the variance of the reference period, but, at the contrary, they are not able to extrapolate outside the variability of the reference period.
We considered 20%% as the limit of agreement because that is the approximate variability of the reference method [28].
The minimum number of batches that should be tested depends on the variability of the reference product and on the assay variability.
We considered a ± 20% tolerance because it is approximately the variability of the reference method [ 12- 14].
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