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Open image in new window Fig. 1 Photograph of SMJ dark color liquid Table 1 Physicochemical properties of SMJ sample Properties of SMJ Color, ICUMSA units 232 Brix 24.8 Polarity 16.5 Purity 66.53 Ash content 0.13 Conductivity (µs/cm) 594 pH 4.3 Turbidity 706 Open image in new window Fig. 2 Cyclone outlet ball valve sample collector (a) and the huge PACW (b, c).
A PCR-positive valve sample taken from a patient with Staphylococcus aureus endocarditis was used as a positive control.
The delay in primary isolation was 2 weeks for the heart valve sample and 8 weeks for the blood sample.
DNA extraction from each valve sample was performed by using Nucleospin Tissue extraction kit (Macherey-Nagel, Hoerdt, France) according to the supplier's recommendation.
In 2012, we received a cardiac valve sample from a patient in Cayenne with Q fever endocarditis who had undergone surgery in Martinique.
For 1 of these patients (patient 13), genotype 7 was detected in a heart valve sample at the time of diagnosis in 2002, but genotype 101 was detected in saliva and fecal samples in 2011 (Table 2).
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Cardiac valve samples were collected in RNase-free tubes containing RNAlater, a stabilization reagent.
The study consisted of a retrospective series of heart valve samples from 15 Caucasian patients hospitalized because of clinical suspicion of IE [35].
As controls, we used cardiac valve samples from 12 patients (10 males and 2 females, median age: 61.8 years, range: 31 83 years) with pre-existing cardiac diseases.
Serum samples were transported at ambient temperature and valve samples were transported at –80°C.
Thirty-seven valve samples underwent fixation by formalin and were paraffin-embedded.
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