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Threshold cycle (C T ) values were established using the ExpressionSuite software version 1.0.1 (Life Technologies).
Four of the six strategies ignored season, and cutoff values were established using the 5000 all-seasons samples.
For both TaqMan Array Cards and single assay results, quantification cycle (Cq) values were established using version 1.0.2 of the ExpressionSuite software package (Life Technologies).
Threshold values were established using the HSI color model for Gormori's trichrome- and Alcian Blue-stained images, and the RGB color model for immunostained images.
Research hypotheses (H1) were directionalised, and as a consequence, critical values were established using one-tailed tests (i.e., for strength performances (N) H1: μpost > μpre; for sum of skinfolds (mm) H1: μpre > μpost).
An independent sample t-test was used to test the H0 of no difference between the means for the measured strength performances and sum of skinfolds for the two training groups after the training intervention; H0: μ1set = μ3set = 0. Critical values were established using two-tailed tests.
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P-values were established using an unpaired two-tailed Student's t-test.
Prior to un-blinding the sample codes, cut-off values for seropositivity were established using previously defined cutoff values.
The (Δ k) statistic derived from the second order rate of change of the likelihood function (Evanno et al. 2005) provided an improved estimate of the mode of true (k), and the mean Q values for each population were established using the full search algorithm in CLUMPP (Jakobsson and Rosenberg 2007).
Equations to predict 17β-estradiol, estriol, estrone or equol Kd or Koc values at the regional level were established using either soil properties (Partial Least Squares (PLS) regression) or one single estrogen (ordinary least squares regression).
The cutoff values for hyper- and hypomethylation were established using the minAS method [ 39] and considering the bimodal distribution of b-values.
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