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The determined binding affinity values were confirmed using a fluorescent competitive binding assay and isothermal titration microcalorimetry.
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Statistical significance of RT-PCR values was confirmed using the non-parametric Wilcoxon test.
Proper calibration of signal intensities in the full range of gene expression values was confirmed using hybridization of different RNA amounts to microarrays (see details in Section 2.2).
Each individual expression value was confirmed using the standardized gene expression values described above.
The N target values of the samples were subsequently normalized such that the mean of the N target values of the eight normal breast samples would equal a value of 1. Target gene mRNA levels were confirmed using an additional endogenous RNA control for normalization; that is, the gene PPIA coding for the peptidylprolyl isomerase A (cyclophilin A).
These results were confirmed using ITLC assay.
Clones were confirmed using NcoI restriction endonuclease.
Results were confirmed using qPCR analysis.
The high Jc value of 1.9 MA/cm2 was confirmed using the precursor films fabricated at a high traveling rate of 10 m/h.
The independent prognostic value of miRNA-126 was confirmed using a Cox regression analysis (hazard ratio=0.49, 95% confidence interval=0.29 0.84, P=0.009).
This result was confirmed using M/z values calculated using the Sauerbrey equation and Faraday's law, which showed the presence of cobalt hydroxide in the electrodeposits.
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