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During training, the range of σ values was set as [0.1, 2] with a step size of 0.1.
Considering the possibility that the first value is an outlier, the median of the first 30 data values was set to zero.
The cut point to obtain the truncated sum of singular values was set to t = (M/2)/2 in our simulations.
Spots with a mean intensity below their local background were excluded and the normalized M value for missing values was set to zero.
Significance for all values was set at p < 0.05.
Statistical significance between mean values was set at P < 0.05.
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(Missing values were set to 0).
The segmentation values were set at 0.7/1/250.
Undetermined raw Ct values were set to 40.
Any resulting negative values were set to zero.
The segmentation values were set constantly at 0.7/1/250.
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