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The values of zone of inhibition for bacterial are revealed in Table 1.
The assays were performed in triplicate, and mean values of zone diameter were taken.
Then it was incubated for 24 h at 37 °C and the experiment was repeated three times, and average values of zone of inhibition was recorded in mm for antimicrobial activity as described before [14, 15].
The effective diffusivities of sintered zinc sulfide pellets calculated by using the values of zone thickness measured in the present study compare well with the literature values reported from kinetic studies.
According to the values of zone diameter and percentage of the inhibition (zdi, % I, respectively), four groups and subgroups of bacterial strains and three groups of fungal strains were characterized by their sensitivity levels to Eucalyptus oils.
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The response surface graph showed maximum predicted value of zone of inhibition for S. aureus (19 mm) at 40 °C, with an extraction time of 40 min at 15% concentration.
The response surface graph showed that the maximum predicted value of zone of inhibition for K. pneumoniae 1 is 33 mm, when 15% extract was prepared at 50 °C for 30 min.
Furthermore, the microhardness values of interface zone were much higher than that of AZ91D substrate.
To evaluate the correlation between the antibacterial activities and the essential oils of the eight Eucalyptus species, all the mean values of the zone diameters inhibition were subject to the PCA and the HCA analysis.
For environmental isolates, the mean values for zone of inhibition were 10.71 ± 1.38 mm (range 8 12 mm) and 27.71 ± 1.603 mm (26 30 mm) for amphotericin B and voriconazole, respectively.
Given the critical resource values of riparian zones, additional data are needed to understand interactions between fire and riparian ecosystems, and how riparian zones affect spatial and temporal patterns of fires at the landscape scale.
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