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The total energy values of the native IFN-CSP and mu-IFN-CSP systems were − 8.8 × 104 and − 9.0 × 104 kcal/mol, respectively.
After 2 h application of 14S24 on the skin disrupted by lipid extraction, the permeability decreased significantly almost to the values of the native skin.
The Km values of the native enzyme and both mutants were roughly identical and were all around 0.1 mM (Fig. 3A).
The RMSD values of the native and mutant structures were significantly similar for both RpoS and TviE, suggesting similar levels of protein folding alterations.
The maximum number of carbon atoms (x(C)) for any MS/MS fragment is calculated by dividing the difference of the measured m/ z values of the native and the corresponding U-C-labeled precursor ions by the exact mass difference of C and C (e.g., 1.00335, Δ m/ z (C, C) for singly charged ions).
Experimental data were converted to the unfolded fraction using fU = (y - yN / yU - yN), where y is the ellipticity value at a given denaturant concentration, and yN and yU are the values of the native and unfolded protein, respectively, extrapolated from the pre- and post- transition base lines defined by the Santoro and Bolen equation [ 44].
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The impedances are normalized to the static value of the native state Z real (0).
Due to low-acid value of the native oil, single-step direct transesterification procedure was followed.
In addition, the ecological value of the native vegetation and its role in sustaining native wildlife were not diminished.
Figure5 reports the Nyquist plots of human OR 17-40 withethe impedance normalized to the static value of the native state.
If this relative marginal value is allocated to forest visits, the amenity value of the native component could be at least €35 million per year despite its relatively small area.
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