Forty or more glomeruli were examined on each slide and assigned values of staining intensity from 0 to 3+.
30 The mean values of staining scores and standard deviations were calculated for vaccinated and non-vaccinated animals and compared using the Mann–Whitney U test.
Furthermore, by multiplying the values of staining intensity and relative abundance of positive cells, we found that the mean staining score of ZDHHC2 in tumor tissues is significantly lower than those in peritumor tissues (P = 0.015)).
The score was calculated by multiplying the mean value of positively stained cells and the mean value of staining intensity.
38 In the present study, however, we did not find a prognostic effect of lumican expression in the tumor stroma, and the prognostic value of staining in the tumor cells was restricted to stage II MSS tumors.
The additional prognostic value of stained TILs, CD8+ TILs, FOXP3+ TILs, and CD8/FOXP3 ratio should be discussed because there have been several studies which find the significance of unstained TILs in patients with TNBC [ 8, 10– 13].
The respective sensitivity and false-negativity values of CK7/20 staining for Barrett pattern in Group A were 10%and90%0%, respectively.
An H-score value was derived for each specimen by calculating the sum of the percentage of cells for both MWCNT and EXMWCNT, which were stained at each intensity category, multiplied by their respective score, using the formula H-score = ∑Pi (i + 1), where 'i' indicates the intensity of staining value between 1, 2 or 3 corresponding to weak, moderate or strong staining, respectively.
The intensity of NFP and MBP immunostaining developed in cerebrocortical axons was assessed microdensitometrically by calibrating the image analyzer taking "zero" as the background developed in sections incubated with a nonimmune serum and "250" as the conventional value of maximum intensity of staining.
Gray value of LRP4 staining at the NMJ (defined as signal) and within the sarcoplasm (defined as background) of the same myofiber was separately measured using ImageJ.
To study the predictive value of EPHA2 staining on overall survival, we selected first biopsy and/or resection samples only; diagnostic biopsies had not been exposed to pre-operative chemotherapy while resection samples had been exposed to pre-operative chemotherapy.
