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The following PD measures/endpoints will be used: successful seizure control (by anti-epileptic medication), adequate sedation or pain control by newborn stress scales (sedatives), adequate treatment of perinatal infection shown by negative repeat blood culture and correlation with MIC- values of cultured microorganisms.
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However, the value of cultured RPE depends on its ability to recapitulate functional and genetic characteristics of the native tissue.
Results showed that Na-F and sucrose Pe values of cells cultured with CSC-HC were less than 40% of those cultured with CSC-cbR, and that TEER values were more than 10-fold higher in cells cultured with CSC-HC compared with that of CSC-cbR.
By contrast, the RMS values of HUVECs cultured on collagen films treated with collagenase for 5 min increased because of abnormal cell deformation.
However, the optical density (OD) value of the cultured sample increased with the increase in initial KL concentration.
The RMS value of HUVECs cultured on normal collagen films decreased after 24 h of seeding because the cells adhered to the ECM substrate.
Figure 2F shows that the RMS value of HUVECs cultured on collagen film slightly increased after 6 h, and then rapidly decreased in the time period (t) from 6 24 h.
The value of any cultured cell as a disease model or as a source for cell therapy transplantation depends on the fidelity and robustness of its expansion potential as well as its ability to maintain a normal genetic and epigenetic status (Pera, 2011).
Moreover, the lactate production and glucose consumption values of the tissues cultured indicated that a stable steady-state was achieved after 6 days of co-cultivation.
We note that our experimental measurements yield D values of ~8 10 for cultured epithelial cells [ 14].
The in vitro IC50 values of these primary cultured cells from cases 6, 8 and 9 were 2.0, 5.8 and 0.4 nM, respectively.
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