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Average PIC value per primer combination was 0.31.
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The number and average of amplicons (alleles) per primer pairs and the polymorphism information content (PIC) value of each loci were estimated using Power-Marker version 3.25 (Liu and Muse 2005).
It was found that two to seven alleles could be detected with a mean of 3.5 per primer pair and the polymorphism information content (PIC) values of these primers ranged from 0.000 to 0.825 with an average of 0.500.
The MI values ranged from 26 to 34 with an average of 30.75 per primer combination.
Amplification of multiplex pools was performed with total primer concentrations of 2 µM (15.9 23.3 nM per primer).
Each pool is normalized to have an average of 10,000 reads per primer pair.
Using 33 selected SCoT primers 273 bands were generated with an average of 8.27 bands per primer among the 50 accessions, of which 208 (76.19%) were polymorphic.
Using 13 designed CDDP primers, 152 bands were generated with an average of 11.69 bands per primer among 64 accessions, with 149 (98.03%) being polymorphic.
Total 82 bands are produced by all 15 selected primers with an average of 5.5 bands per primer.
Two to three PCR replicas were used per primer set.
On average, 18 markers were produced per primer combination.
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