Exact(3)
In terms of level, the value of the technical coefficient coincides or is close to that of the attractor point.
The r2 value of the technical replica experiment performed probing two separate bead-arrays with the same RNA sample was 0.9876.
For each gene of interest, the mean Cq value of the technical and biological replicates in the 2 groups being compared (S v C, FR v C, FR v S, or S v FR) were determined.
Similar(57)
The validation results indicated that the pressure waves propagated into the soil as hemispherical waves, and the peak pressure values closely matched the predicted values of the technical design manual TM5-855-1, except for large distances.
To test the programs' precision, we assessed whether the expression values of the technical replicates correlated better before or after batch adjustment, first between the two replicates from the VAS data using Pearson's correlation coefficient, then among the three SMRI replicates as assessed by intraclass correlation (ICC).
The relative luciferase levels were calculated as follows: first, the background was subtracted from each value, and then the average values of the technical triplicate were calculated.
Initially, logarithmically normalized exonic read density values of the technical replicates sequenced from the oestrus and dioestrus cDNA libraries from uteri with and without bacteria were shown to be consistent by regression analysis performed in R (version 2.15.2).
Raw microarray data were normalized and statistical comparisons were performed using Array Studio (Omicsoft Corp .. Fold changes were calculated based on the mean values of the technical replicates for each probe and adjusted P-values were calculated using the Benjamini and Hochberg method with a false discovery rate α-value of 0.05.
After taking the mean values of the technical replicates for all remaining features, we then created separate files by tissue type and, within each file, eliminated any features with more than 10% missing samples, imputing the remaining missing values using the emArray method in the LSimpute package (Bo et al., 2004).
All of these genes also had a SD of the Ct-values for the technical duplicates of ≤ 1.41 and a SD of the raw Ct values of all samples ≤ 1.0.
Though not shown in the work-flow, ReadqPCR also allows the user to deal with technical replicates by (optionally) calculating the arithmetic mean or median of the raw Cq values for the technical replicates of a given gene.
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