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This behavior reverts to baseline when the aversive stimulus is switched off, and is blunted by treatment with the anxiolytic drug Diazepam, demonstrating predictive validity of the assay, and indicating that the observed behavioral response has a significant anxiety component.
The strength of the preliminary evidence, the prevalence of the marker, the reproducibility and validity of the assay, and the feasibility of real-time marker assessment play a major role in the choice of the design.
The validity of the assay was examined against 22 reference strains within the family Pasteurellaceae and 17 field isolates (nasal) that had been collected previously from feedlot cattle and tentatively identified as M. haemolytica based on morphology and substrate utilization.
As well, specificity of the antibody to the analyte of interest is the limiting factor in the validity of the assay.
The validity of the assay was established by testing a diverse set of rice varieties and comparing the results against those obtained using standardised in vivo procedures performed by two highly experienced testing agencies.
Controls were included for each RPA analysis to confirm the validity of the assay (data not shown).
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However, given the validity of the assays used, this difference is likely minimal.
The other two, however, relate to the validity of the assays themselves: that is, the assays do not distinguish between apoptotic and necrotic cells.
The other three problems described here relate to the quality of the assays performed rather than the validity of the assays themselves.
Serological evaluation of KSHV has not been standardised (Pellett et al, 2003) but the highest seroprevalence observed in Italy and the increasing antibody titres with age reinforce the validity of the assays.
The validity of the assays was confirmed by accurate quantification of viral copy numbers (one copy per cell) from SiHa cell line, which is known to contain one HPV-16 copy or two per cell.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com