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Internal validation was successful with polytomous c-indexes between 0.64 and 0.69.
Because model validation was successful, we updated the model on the pooled data (n=5909) to make full use of all available information.
For 18 of these mitogenic classifier genes the RT-qPCR validation was successful and showed a highly similar trend for insulin, X10 and IGF1 conditions (Fig. 4b).
The trait-stratified GWAS screening approach followed by selected validation was successful, and we report seven novel loci which are strongly associated with autoantibodies and serum IFN-α, two important heritable subphenotypes in SLE.
qPCR validation was successful using a different set of individuals from the same cross in a different environment and year from those used for the microarray, indicating that the differentially expressed genes had relative expression levels consistent across different growing conditions and years and between different groups of individuals from the O3 × R5 F1 population.
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Three of the four methods are fully validated and the validation is successful.
If this independent validation were successful, a prospective validation of the miRNA candidate biomarkers would be warranted.
Validation is successful if qRT-PCR results fulfil these criteria for at least one subtype of NSCLC (AdCa or SCC).
Overall, qRT-PCR validations were successful for thirteen out of fourteen sRNAs.
The validation strategy was successful thanks to the high proportion of PCPs who returned completed validation questionnaires (i.e., 90%).
The first time we validated with 2006 data, the validation was not successful.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com