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Therefore, our findings will require careful internal and external validation to confirm validity.
In addition to Q-PCR validation, to confirm that specific members of the community were truly present, a pair of primers designed to detect Veillonella parvula (based on PhyloChip probes; VDF5'-CGTAATCAA CCTGCCCTTCAGAGG -3', VDR 5'-TTTCTGGCTTCC GAAGAAGAGGAAC-3') was used to amplify a PCR product which was then submitted for bi-directional sequencing.
The experimental validation to confirm if these proteins are functional is yet to be done.
TALA, having the highest affinity and content, was isolated for further in vitro pharmacodynamic validation to confirm the screen results.
Finally, our findings require validation to confirm the utility of Prx4 in type 2 diabetes risk prediction.
Using two complementary methods, aCGH and qPCR, we performed experimental validation to confirm individual copy number variants.
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Potential weakness of the majority of exiting algorithms, e.g., Refs. [5, 6, 7, 8, 9, 10, 11], is that they need either human-made instruction/intervention or an off-line cross-validation to confirm the value of threshold before operation, and thus make them largely limited in real applications.
Overall accuracy, coverage, false positive, and false negative rates were tabulated first using all 265 test sequences, then re-calculated using 10-fold stratified cross-validation to confirm that results were not significantly different within any subset of the data.
The expert reviews and usability evaluations were conducted as an internal validation method, and a field evaluation was used as an external validation method to confirm the feasibility and educational effects.
In addition, we describe in vitro validation experiments to confirm the effectiveness of the miRNA sponges.
To ensure data quality, you could use Excel's data validation features to confirm that each retail outlet had an employee assigned as the customer's main contact.
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