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Table S1 provides comprehensive data from both the primary and validation screens, and Table S2 provides a list of the 128 validated genes with descriptions.
The validation screens at the DCC were maintained in English for standardization purposes.
Validation screens were performed analogously, with compound concentrations: 100 nM, 1 μM and 10 μM.
Primers used for the validation screens of ELF3, GNAS, KLF5 and ERBB3.
In addition, PMM can potentially be used to gain power for secondary validation screens.
In the validation screens three different concentrations of the drugs were used.
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All DMRs positively validated in the microarray validation experiments were subjected to initial clinical validation screening.
The comparison of the first and second rows in Table 1 ("Currently unemployed" and "Valid matches") illustrates the combined impact of attrition, nonresponse, and the matching validation screen based on demographic characteristics.
Here, the 74% reproducibility rate is comparable to the average reproducibility rate observed for the validation screen when the same collection of dsRNA was used to self-validate, which clearly demonstrates a limitation of experimental validation [ 26].
A secondary functional validation screening revealed that the potential oncogenes participate in different cellular processes affecting proliferation, cell viability, apoptosis or differentiation.
For example, one sample in the Validation Screen, 4198, exhibited nine coding region mutations and another, 6985, six mutations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com