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The system was validated with a 75% validation rate in maize [ 13].
SNVs and CNVs were validated by Sanger sequencing (93% validation rate) and genomic qPCR (96% validation rate), respectively.
Selected CNVs were further experimentally validated, achieving a 73% PCR validation rate, which is considerably higher than the previous validation rate.
Samples were validated in lung tissue achieving a validation rate of 65.7% (Supplemental Table S3).
Unique-to-GATK indels had a validation rate of 180 of 336 (54.0%), being validated.
Full consensus predictions had a validation rate of >98%, but some partial consensus predictions validated too.
Only six panned out — a validation rate of eleven per cent.
The CREKB showed the highest validation rate at 11%, closely followed by the targeted drug screen (9%).
We demonstrate how incorporation of the results of GESS analysis can enhance the validation rate in RNAi screens.
Despite known limitations of mass spectrometry to detect protein expressed at low level, we estimated a 14% validation rate.
Although the validation rate was low, a number of drug gene interactions were successfully identified and are now being investigated for protein induction and in vivo effect.
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