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The availability of genome-wide sequencing data will help to establish consensus guidelines for the identification and validation of true CNVs.
Noteworthy, the downregulation of vitellogenin genes in diapausing T. uticae females was considered by Kawakami et al. (2011) [ 14] as a validation of true diapause.
Ideally, mapping time is largely spent on the matching and validation of true alignments, but if the seed weight is insufficient, the examination of random hits will dominate the running time.
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In addition, standardization of research protocols and assays going forward may be helpful for combining data across multiple cohorts in order to meet the stringent power requirements of GWAS and also to facilitate validation of true-positive findings.
Another mandatory step in the development of CEC assays is the validation of the true endothelial origin of cells designated CEC by that assay.
These differences may be due in part to use of nonspecific antibodies to assay ER β and some studies of archival specimens lack validation of a true TNBC phenotype [ 17, 20, 21].
Second, unlike previous comparative studies using transcription factor binding data (Additional file 1: Table S1) or "spike in" experiments [ 31], we do not have external validation of the true enrichment regions, which prohibited us from assessing detection sensitivity and specificity.
To asses the performance of the tested methods we counted in cross-validation the number of true positives (TP), true negatives (TN), false positive (FP), and false negatives (FN) with respect to either one of the two classes.
In addition, the ability to unambiguously identify the precise phosphorylation site was recorded (pass/fail, phosphosite validation), allowing the determination of true positive and false positive rates for the automated analysis.
We compare QSAR and PCM methods, multiple algorithms (including DNNs), the differences between binary class and multi-label models, and usage of temporal validation (effectively validating true prospective use).
Mr. Matthews, who is expected to move on to other high-technology ventures, said "the union of Alcatel and Newbridge is a true validation of our strategy".
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