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Standard deviation and percentage error were investigated for validation of the experiments.
Extraction temperature of 70 °C, ultrasound power of 70 W, time of 23 min and solid liquid ratio of 1 10 g/ml were determined as optimal conditions with a maximum polysaccharides yield of 16.21%, which was confirmed through the validation of the experiments.
JHI participated in performing the microarray hybridisations and advised on RT-qPCR validation of the experiments.
Though the simple description of the enrichment as a list of most frequently observed aptamers in the data set is sufficient for conventional validation of the experiments success through concrete binding experiments, a better way of description has to be found when aiming at the improvement of prospective SELEX runs.
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This study presents a statistical validation of the experiment based on a standard 2k factorial experiment design and the resulting empirical model of the enhanced hydrogen producing process.
After validation of the experiment using the optimum process parameters generated by the Design Expert software, the l-DOPA production was observed in the medium before optimization and after optimization.
The results of this time-course analysis confirmed results of the CATMA microarray, thus constituting further validation of the experiment for these four additional genes.
Expression of the ACME element was also increased in USA300 after infection, compared with USA400; this was expected because ACME is only present in USA300 but it serves as a validation of the experiment.
For validation of the siRNA experiments, RNA was extracted from at least three separate transfection experiments for each gene by using the Qiagen RNeasy Mini kit, and 1 μg of total RNA was reverse transcribed by using SuperScript II (Invitrogen) and oligo dT) primers.
A quantitative validation of the microarray experiments was performed by real-time RT-PCR.
In order to provide independent validation of the original experiments, we treated new cultures of cells and again collected conditioned medium using the two drug concentrations, 5 × 10−6 M and 1 × 10−5 M.
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