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However, stronger validation of the effects on PA using objective measurements is recommended [ 63].
Functional validation of the effects of these mutations could contribute to understanding the roles of these factors in regulating constitutive splicing.
Note however that there is presently no direct experimental validation of the effects of the Koski-Golding demonstration on the prediction accuracy of BLAST reciprocal-best-hits (or even one-way best matches) approaches.
We therefore conclude that genome-wide prediction methods have the potential to detect favorable alleles, but a validation of the effects in subsequently conducted well-designed trials with a reduced set of lines is mandatory.
The validation of the effects of molecules correcting the aberrant splicing caused by the IVSI-6 mutation can be performed in vitro using erythroid precursor cells isolated from these transgenic mice, as well as in vivo following administration of splicing correctors, as performed with different in vitro and in vivo experimental systems by several research groups [ 45– 50].
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A validation of the effect of the GNRs induced local heating on the DNA, was achieved by performing a control experiment by varying the sample temperature from 25 °C up to 95 °C and monitoring the absorption spectrum (Fig. 6a).
HIFs (Heterozygous Inbred Families) were selected from progeny of RIL 173 (Sha x Col population, Figure S6) for the validation of the effect of QTL5.
Validation of the effect using individuals not included in the GWAS is necessary in order to confirm the predictive ability of these markers.
(G ) Validation of the effect size of Klf7 perturbation of knockdown (left) or overexpression (right) on other drivers in bone-marrow hematopoietic stem cells (y -axis).
These findings support the involvement of the EVC2 mutation in microtia-associated malformations, although formal proofs needs to come from the identification of additional patients with the mutation and further functional validation of the effect of the mutation on the EVC2 protein.
While the experimental model used in this study does allow validation of the effect of cancerous cells on non-cancerous tissues, the reliance on medium as a conductor between the adherent cell monolayers and the tissue rafts is not ideal for understanding progression of CFE in-vivo.
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