Specifically, "To infer a rate of validation of the SNP discovery, it would be useful to see a small subset of identified SNP (10 20 should be sufficient) randomly chosen from the discovery set genotyped using a different method (e.g. Sequenom) in the discovery animal DNA, and ideally a small cohort of animals of the same breed".
Computational target prediction combined with a comprehensive functional validation enabled the discovery of a set of twenty putative target genes for all productivity enhancing miR-30 family members.
The loadings of the validation PCs, calculated independently of the discovery PCs, were highly and significantly correlated with the discovery PC loadings, showing strikingly similar patterns.
For the identified PAK7 risk duplication, we performed standard qPCR validation of the CNV calls in the discovery sample (Supplementary Material, Table S4).
For validation of the model found in the discovery cohort we performed linear regression analysis for the predictive value in a second cohort of patients (validation cohort).
For validation of the results emerging from discovery cohort (TCGA_Array) we used data from two publications.
Scientists across the nation will have ready access to MICCoM-developed open-source software tools for simulation, data handling and model validation, leading to the discovery of new materials for advanced energy technologies.
Although the biological role of IG transcription suggested in the study is only a hypothesis and will require further experimental validation, the discovery of the potential role was significantly facilitated by the collected information and the tools provided in SKB.
We investigated the validation performance on the basis of the discovery platform marker coverage, the predicted variant size and the allele frequency as evaluated in the discovery dataset.
The clinical and pathologic characteristics of the discovery, validation, and neoadjuvant cohorts were extracted from the clinical records by chart review (Tables 1 and 2).
