A key step necessary to begin to resolve these discrepancies between theory and empirical observations requires the validation of fitness consequences of variation at specific loci or pathways in the field (Turelli and Barton, 2004; Fournier-Level et al., 2011; Hancock et al., 2011).
Whether AKT represents an important survival signal and is an appropriate target for molecular-based therapy awaits validation of the incidence and clinical consequence of AKT activation in pancreatic cancer specimens.
We also provide important biological validation of the association data: a physiological consequence of the TAS2R9 polymorphism (i.e., a loss of response to several ligands).
As a consequence, validation of the method is based on comparison of measured values of EVLW with gravimetrically determined EVLW.
Partly as a consequence, validation of the microarray data gave variable results, from perfect match, to opposite changes in a few, although effects observed in the microarray, with fold changes as low as 1.2 (e.g., NADH1 and PA2G4) were validated by RT-qPCR.
In all cases the identification was unambiguous (Table 2 and Figure 4), and in consequence the validation of the samples with the nuclear ITS1 was not necessary.
In consequence, the validation of the clinical relevance of nsSNP spatial interactions is limited.
As a consequence, the validation of the approach by means of Northern and Western blots concerns the comparison of relative values.
An important aspect in the validation of the p53 MDM2 interaction as a cancer target is assessing the consequences of p53 activation in the normal proliferating tissues.
And what of the consequences?
