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Workshop participants explored the development and validation of techniques for extrapolating subcellular or in vitro measurements to whole body biotransformation rates or enzymatic activity rates across fish species that could then be used as stand-alone bioaccumulation assessments or incorporated into BCF and BAF model predictions.
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In the last decade, this reapplication of older techniques and the validation of existing techniques for measuring neutral winds in the MLT region have emerged.
Common molecular biology techniques have never been carefully tested according to the basic principles of validation of quantitative techniques.
This provides some validation of our techniques.
Validation of such techniques has several difficulties.
Although a potentially powerful forensic tool, knife mark analysis is still in its infancy and the validation of imaging techniques as well as quantitative approaches is ongoing.
We have recently published validation of this techniques against other gold standard methodologies [13].
While our approach can provide location estimates with confidence intervals based on the data model, there remains the need for independent validation of the techniques with known locations.
Ideally, after normalisation, replicates should be approximately the same, so the distance between them is a criterion widely used for validation of normalisation techniques, e.g. [16].
Research into SLNB and USPIOs is promising but at present validation of these techniques is still incomplete.
There is, however, a need for further validation of these techniques in larger patient cohorts with adequate follow-up periods.
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