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Biosensors based on whole-cell bioluminescence have the potential to become a cost-effective alternative to conventional detection methods upon validation of target selectivity and sensitivity.
Validation of target cleavage by RLM-5'RACE was done as described previously [27].
Our qPhage strategy allows fast and precise validation of target tissue-specificity in the homing of selected phage or in large-scale evaluation of dozens of samples, which is particularly appealing for studies in vivo, including patients [29] [30].
Gapdh was used as an internal control for qPCR validation of target mRNA genes.
PLK conducted the RWPE1 experiment, performed statistical, functional and cluster analysis of microarray data, conducted validation of target genes using QRT-PCR and prepared the manuscript.
For experimental validation of target cleavage, total RNA was extracted from soybean roots at 0 and 3h after B. japonicum inoculation.
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Therefore, the aim of this work is the implementation and validation of target-biased scoring methods within the open-source docking framework ParaDockS [2].
A pharmacologist's training encompasses the entire spectrum of the drug discovery process: identification and validation of targets, study of preclinical animal models, determination of dosing schedules, and testing of toxicity in clinical trials.
Therefore, validation of targeting effects for a given ligand may be difficult to resolve with traditional competition–inhibition methods using an excess of free ligand or simple models via positive negative cells (Yu et al. 2010).
Three week old seedling tissue was used for validation of targets.
Naturally, we wanted to focus on these 29 unique genes and further validate the GTI method by examining the value of these candidates in the cancer biology of glioma (see section on validation of targets below).
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CEO of Professional Science Editing for Scientists @ prosciediting.com