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Primary and secondary antibodies and validation of specificity are listed in Table S2.
Indeed, we would suggest that the use of this rabbit polyclonal HOXA4 antibody in these types of applications is unwarranted unless definitive validation of specificity is presented.
Under optimized working condition, the two-tube assay revealed good specificity without nonspecific amplification using size detection system though conventional validation of specificity is usually done by probe detection, hybridization, nested PCR, and sequencing.
(b) Validation of specificity, showing lack of effect on the non-target mRNAs (as indicated) after transfection of vascular smooth muscle cells with the indicated siRNAs (n/N = 2 3/4 6).
Similar(55)
In addition, method validation results of specificity, linearity, accuracy, precision, lower limit of quantitation (LLO Q, and carry-over demonstrated that this on-line sample pretreatment HPLC/MS system was robust and valid as a practical assay of drugs and metabolites in the biological samples.
Immunostaining with specific antibodies and validation of antibody specificity have been previously reported for this cohort of patients.
This method enables cellular validation of the specificity of small molecule DUB inhibitors.
Validation of probe specificity and optimization of hybridization conditions were conducted using fluorescently labeled 16S rRNA gene PCR products of pure culture strains.
Given the canonical role of IKKβ as part of the IKK complex, this finding was considered a strong validation of the specificity and usefulness of the screen.
Hence, validation of antibody specificity for ICW using WB may prove most useful where the antibody recognizes a continuous epitope that is fully unmasked during fixation.
Although the association of the luminal phenotype with Tamoxifen and ESR1 expression level provides an additional validation of the specificity of the methodology, a more pressing question in the context of breast cancer therapy is the basal phenotype since effective treatments for this group of patients are limited.
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