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Functional validation of individual genes associated with root development through knockout or gain-of-function mutants still holds merit.
In addition to the validation of individual components of the dynamic growth model, overall validation of the whole model was carried out.
Quality assurance procedures were designed to quantify both the dosimetric and positional accuracy of respiratory-gated IMRT, including film dosimetry dose measurements and Monte Carlo dose calculations for verification and validation of individual patient treatments.
Hence, validation of individual gene targets is essential to confirm their functional relevance to the cancer cell phenotype.
Further functional validation of individual proteins is ongoing and might provide new insights in PCa progression potentially leading to the design of novel diagnostic and therapeutic strategies.
Because of the potential to design tailor-made inhibitors of the Plasmodium redox network as innovative antimalarial drugs [4], [5], target validation of individual redox-active enzymes paves the way for future drug discovery approaches.
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Without experimental validations of individual Oct3/4-target genes, the utility of genes identified by searching octamer-binding motifs are limited at this point.
Whereas this clearly demonstrates the power of the PBPK/PD modeling approach, a full quantification of the quality of fit and predictions of the model presented here in comparison to state-of-the-art model implementations of the GIM is difficult as no validations of individual model predictions of full-day trials are published, but only pure predictions without data or only fitted trajectories.
An expert consultation meeting conducted in 2000 (OECD 2003) discussed issues on validation, especially of individual test components versus the whole DNT test method.
Logically, a combination of biomarkers could enhance diagnostic accuracy, but validation of current individual candidate biomarkers is still underway.
Two aspects are considered essential for the good final performance: The use of separately determined, product-specific single-particle drying kinetics as a basis for every scale-up duty, and a stepwise methodology of model development with detailed experimental validation of every individual step.
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