Sentence examples for validation of hpv from inspiring English sources
The combination of p16 immunohistochemistry and HPV PCR testing could lead to improved validation of HPV positivity.
Direct automated DNA sequencing is a reliable means for validation of HPV genotyping in a routine clinical microbiology laboratory.
In the last decade, the clinical importance of better understanding tumor biology in HNSCC has emerged through validation of HPV status as a significant molecular predictor of patient survival and recurrence.
However, further development and validation of HPV detection in oral fluid is warranted due to the low sensitivity and specificity for clinically relevant HPV infection before being implemented as a clinical diagnostic medium.
Identification of a signature DNA sequence by the automated Sanger method is useful for validation of HPV genotyping and for molecular testing of C trachomatis and N gonorrhoeae in liquid-based cervicovaginal Papanicolaou (Pap) cytology specimens for clinical laboratories with experience in molecular biology to increase the specificity of these DNA-based tests.
The introduction of the low-temperature PCR system with a robust DNA polymerase, which does not require sample purification or DNA extraction, has simplified the process to direct DNA sequencing procedure, making rigorous validation of HPV genotyping in a clinical laboratory economically sustainable [ 26- 28, 30].
Some European countries [ 33, 34] adopted the Meijer et al. criteria for clinical validation of the HPV DNA test.
By following CAP (College of American Pathologists) principles, the validation of Tellgenplex™ HPV DNA Test were conducted compare to HCII in the molecular biology lab of KingMed, and the correlations that we detected were found to be (coincident rate = 90.5 %, Kappa = 0.88).
A 92% CIN 3 detection rate has been proposed as the clinical sensitivity along with an 85% CIN 3 confirmation as the clinical specificity for validation of all HPV assays [ 14].
The high proportion of women that were HC2-positive but HPV-negative in genotyping suggests that HPV genotyping may be useful also for validation of results in HPV screening.
Assessment of emerging HPV tests in this context is essential, using well-annotated samples, such as those generated via the Validation of Genotyping Tests-HPV (VALGENT) framework.
