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Clearly more efficient methods for accurate, experimental-based annotation and validation of function are needed.
This dataset therefore provided a significant contribution of value for gene discovery and validation of function for the genus.
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However, the human genomics community is currently facing several challenges, including phenotyping, sample collection, sequencing strategies, bioinformatics analysis, biological validation of variant function, clinical interpretation and validity of variant data, and delivery of genomic information to various constituents.
Although widely used for gene discovery and validation of gene function, one impediment to a broader use of transgene-mediated gene silencing especially in crop improvement has been the question of specificity.
We have discussed ongoing community efforts to address these challenges, including clinical-grade sample collection, digitalized phenotyping, acquisition of high-quality sequencing data, rigorous generation of variant calls, comprehensive functional annotation of variants, and biological validation of variant function.
To elucidate these interesting and novel biological features, validation of gene function using stable transformation approach must be helpful.
The efficacy of liposomes bearing miRNA mimics uptake and gene knockdown was similar in both 2D and 3D in vitro culturing models highlighting the proof-of-principle for the applicability of 3D collagen-based scaffolds cell system for validation of miRNA function.
The cDNA information also is indispensable for validation of gene function by reverse genetics.
This is achieved in R by setting the parameter validation of the function plsr to "LO O.
Validation of gene function certainly remains an outstanding problem arena.
(B ) Validation of miRE function by luciferase assays.
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