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Post hoc validation of assignment of downwind and upwind study sites for each testing day was checked using wind rose data.
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As an additional validation of parentage assignment accuracy, known locations of parents during spawning were compared to natal rivers of assigned families for congruence.
Validation of cluster assignment was carried out using R', the adjusted Rand index [ 42- 44].
We also present a novel strategy for automatic validation of peptide assignments based on identification of a peptide by consecutive CID and ETD fragmentation in an alternating mode.
Moreover, by using a slightly longer C to H CP contact time (700 μs), we obtained many CαHN+1 contacts in the 2D CH, which also allowed validation of sequential assignments (see Figure S8).
Predicted sequence-specific chemical shifts are used as a template for re-assignment and validation of existing resonance assignments.
The cross-validation of the assignment of these water molecules using gold-standard half maps can be found in Figure 4 figure supplement 1. DOI: http://dx.doi.org/10.7554/eLife.06380.006 10.7554/eLiFigure80.007 Figure 4 figure supplement 1. Cross-validation of water molecule assignment through comparison of gold standard half maps.
This was used to calculate the estimated probability of presence that gives a statistical validation of the gene assignment.
They also served as a validation of linkage group assignment.
Validation of peptide spectrum assignment was done using PeptideProphet with the "accurate mass binning" option enabled.
A known intron in a similar position in an orthologous gene was considered a validation of correct position assignment.
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CEO of Professional Science Editing for Scientists @ prosciediting.com