Method validation is obviously a highly recommended step.
However, further functional validation is obviously necessary before confirming such hypotheses.
The results of this study stand in need of cross-validation and, obviously, much additional outcome research is necessary to evaluate the efficacy and validity of the findings for screening purposes.
Moreover, validation of IGT obviously requires evaluation of the treatment outcomes that will inevitably require head-to-head comparison with other different medical/surgical/radiation therapies, and also evaluation of complex therapeutic strategies with the combination of IGT and other treatments.
What will validation validate?
Obviously, further validation of this gene set or PoT is needed before considering its potential application as a screening device for identifying IR-inducing pollutants.
The "Array-based bioinformatic analysis on pediatric primary central nervous system germ cell tumors" dataset, previously used for validation and evaluation was obviously too small for building a robust model capable of generalization.
The proposed model has been internally validated (Altman and Royston, 2000), but validation in an independent study is obviously warranted.
Kalani Leifer: I'm still wrapping my head around it, but the thing that has been easy to grasp is the impact it's had on COOP and the whole community, from our full-time team and all of our captains and spread out all the way to our newest apprentices and all our cohorts.We're still scrappy and figuring it out, but I think seeing that validation was pretty special, and obviously for me as well.
Obviously, large-scale validation and functional studies at both mRNA and protein levels are needed to corroborate the role of the key regulatory genes (e.g., ITGAX, VEGFA, FN1, CCND1, CA12, LAMB3, MYL9, etc).
