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For most tissue slide-based in situ miRNA detection, analytical and clinical validation is still required.
Even in advanced cases, recent genetic studies have suggested that the use of targeted therapy may be useful, but additional validation is still required [ 15].
Preliminary results of this approach with a sensitivity analysis of the coupled model are shown but further validation is still required.
However, it should be noted that these results are an internal validation of the developed CHIP-rule and that external validation is still required.
A discriminative threshold PTX3 level ≥1 ng/ml in BAL fluid was identified to help translation into clinical practice, but validation is still required.
However, experimental validation is still required for their binding sites containing the 5-HT3B subunit as many of the residues with predicted ligand interactions in B+A− binding sites are known to not effect 5-HT activation or granisetron binding (Lochner and Lummis, 2010; Thompson et al., 2011c).
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Independent validations are still required to accurately measure the expression of each gene of interest.
Additional studies and validations are still required before transferring any of these therapeutic proposals, here listed, to routine treatment for human subjects.
Further quantitative validation of the coupling is still required.
An independent validation in another cohort is still required before any firm conclusions can be drawn.
However, much work regarding validation of these approaches is still required.
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