We selected 7 targets (Table 1) for validation by considering several factors: the magnitude of the viability change as indicated by normalized luciferase intensity, the number of siRNAs altering the luciferase intensity in the same direction, and any documented involvement of sense transcripts in cell survival and/or proliferation.
Subsequent mutation validation was done by several methods.
We demonstrate the quality of our refined structural models by validation with several commonly-used structural analysis tools.
As previously discussed, the pedestrian validation step is composed by several validators, each one supplying a vote that is then provided to the final evaluation step.
To probe the heterogeneous cellular composition of the P1 cell fraction, we randomly harvested single-cells from the P1 fraction and carried out reverse transcription using the Smart-seq2 method, followed by qPCR validation with several NSC/NPC markers, such as Prom1, Gfap, Dlx2, and Dcx.
This article describes the theoretical rationale, development and validation of several instruments developed by the author and now widely used for high risk occupations and for screening corporate managers and key personnel in occupational settings.
Most of the projects, whether powered by processors or by brains, rely on independent validation of a result by several volunteers.
The performance of this approach was evaluated by several validation indices and compared with multiple linear regression (MLR) and regression kriging (RK).
We have constructed a first generation physical map for the Asian seabass and the reliability of the resulting map was confirmed by several validation methods.
The performance of the JJ risk engine was evaluated by several validation criteria.
As demonstrated by several validation studies, the phototoxic potential of chemicals can be evaluated effectively by in vitro methods [ 36].
