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It is important to note that our RT-PCR microarray validation analyzed only the expression of the two key cytokines IL8 and MCP1 and did not include genes induced specifically by WT-CF compared to ΔPrtT CF. Based on the results of the microarray analysis, we hypothesized that A. fumigatus germinating conidia or CF activate MAPK signaling in A549 cells.
GN designed and carried out the microarray experiments, performed qRT validation, analyzed the data, and wrote the initial draft of the manuscript.
A difference with our test-retest reliability compared to the original validation is that we tested patients at two occasions rather close in time, whereas the original validation analyzed stability over a period of time of a year.
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Tables 4 and 5 report correlation coefficients among the attribute scores and specific other items in the questionnaire that were employed in the construct validation analyze.
This validation study analyzed urine from 74 patients with type 1 diabetes at the baseline visit (visit 1).
The coverage validation analyzes how large a proportion of the molecules from the input database can be processed using the validated EEM parameter set (therefore the validated EEM parameter set covers these molecules).
The coverage validation analyzes, how large a proportion of the molecules from the input database are composed only of the atom types included in the input EEM parameter set.
In the present biomarker validation study we analyzed new larger sets of gender- and age-matched plasma samples (50 MCI and 50 AMC) collected at different sites.
Firstly, the integer ambiguity resolution and validation procedures, especially the Least squares AMBiguity Decorrelation Adjustment (LAMBDA) estimation and RT validation are analyzed.
The inter-assay imprecision (%CV) of the validation samples ranged from 10-23% (Additional file 1, Table S7; n = 3 aliquots for each validation sample analyzed in duplicate).
The inter-assay imprecision (%CV) of the validation samples was 11%, 8%, and 21% for Low, Mid, and High, respectively (n = 3 aliquots for each validation sample analyzed in duplicate).
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