Sentence examples similar to validating transposition from inspiring English sources
Additional file 2 lists 321 independent insertion events identified in the 3 Paracoccidioides genomes, demonstrating integration of the transposon into a TA dinucleotide within the fungal genome and validating genuine transposition.
To validate the putative transpositions of mPing in the S0, S1 and S2 generations of the etoposide-treated rice plants, revealed in the TD analysis, a set of 39 pairs of locus-specific primers each containing an intact mPing (see Additional file 2) were used for PCR amplification at annealing temperatures ranging from 56°C to 60°C depending on the primers.
Practice validating others' feelings.
We did not attempt to validate any of the three transpositions.
To test this hypothesis, we examined if the transposition activity, which has been experimentally validated in Corynebacterium glutamicum29, was also functional in proteobacteria.
This result, combined with the observed reasonable phenotypic penetrance, makes PB transposition an attractive, nonviral approach to validate positionally-cloned gene mutations and screen candidate genes.
In this context, transgenesis protocols based on Sleeping Beauty or piggyBac transposition providing the transposase in the form of mRNA have been validated in many eukaryotic species, including mouse and rat models [ 7- 9], and in cultured human cells [ 10].
We tested each of the different stages of the process, i.e. targeting of specific progenitors, transposition into the genome and generation of inheritable random combinatorial hues, to validate CLoNe.
"I validate those feelings.
