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In fact, our approach goes a step further in validating the sequence by accounting for the reverse complement of the scaffolds, thereby eliminating any potential false rearrangements that might be introduced in the assembly.
DM leads the team which is validating the sequence assembly against a high resolution radiation hybrid map.
Since these HMMs are built using structural alignments, they are highly sensitive in validating the sequence homologue obtained for a superfamily member as a true positive (TP).
As expected, the Cas9/gRNA-mediated mutations occurred at or near the target site, further validating the sequence specificity of this targeting process.
In addition to validating the sequence fidelity of the existing Perlegen and Arabidopsis lyrata genome sequence data, our DNA sequencing data also allowed us to identify a range of LSGs which are Arabidopsis thaliana accession-specific and hence excellent candidates for de novo origins of an LSG within the Arabidopsis thaliana species genepool.
On average, 72.4% and 78.2% of the valid reads in directional RNA-seq data of the chimpanzee cerebral cortex were uniquely mapped onto the chimpanzee and the human genome sequence, respectively, except for random chromosome sequences (Additional file 1: Table S2), validating the sequence similarity between the chimpanzee and the human genome.
Similar(54)
This ORF number was carefully validated by comparing the sequences deposited in three databanks (CADRE (The Central Aspergillus Resource), JGI (Joint Genome Structure) and the BROAD Institute) with the aim of identifying and validating the sequences for probe design.
In ten of the areas PCR and sequencing was carried out to validate the sequence.
This integrated alignment was used to assess and validate the sequence assemblies and to build the physical map further.
In order to validate the sequence of the wild-type strain and to determine the complete MDR and XDR sequences, we independently sequenced three KZN strains (one wild type, one MDR, and one XDR) using whole-genome sequencing.
The identified positions were then manually validated on the electropherogram to validate the sequence.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com