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Model simulations effectively match experimental data, validating the assignment of parameter values described above under "Neurohumoral Control of Pressure-Diuresis/Natriuresis". Figure 8B illustrates model-predicted steady-state renin and angiotensin II activities (ϕ R and ϕ A2) and sympathetic tone (ϕ SN) as functions of Q in for the normal case.
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Only one peak for each of the isomers was observed, validating the assignments.
Immunoblotting also validated the assignment of some of these proteins.
This variable was then used as an external indicator to validate the assignment of samples to the three clusters.
Additional characterization of their binding elements through mutation analyses and reporter assays should further validate the assignment.
In order to validate the assignment of individuals with apparently no spores attached to their oesophagus, we viewed the oesophagus of slightly squashed animals at 400 × magnification.
Consensus decision making exploits the goodness of multiple search algorithms to validate the assignment results of spectral data at a relatively low cost.
The gold clusters clearly pinpointed the C-termini of the Med17 and Med22 subunits at roughly the same position on the upper-right portion of the core Mediator, which directly validates the assignment of the Head module.
The band assignments were made using the asynchronous plot for which the highest spectral resolution was obtained and extrapolated to the synchronous and baseline-corrected spectral overlay for each data set to validate the assignment.
Although we have not experimentally validated the assignment of snoRNA genes to UTRs, these assignments are likely to be correct since we found that snoRNA genes overlapped with protein-coding region of the gene as well as the UTR.
Although the functions of the proteins/unigenes still need to be further validated, the assignment of functions provides important reference for identification of the targets in the in vivo experiment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com