The model was validated at optimal conditions in order to check its adequacy and accuracy and an experimental yield of 64.1 (±0.0) gFOS/gsucrose was obtained.
To validate the optimal conditions for real clinical applications for Ramathibodi Hospital (Bangkok, Thailand), the performance evaluations of the coatings such as cytotoxicity test and color differences were conducted, and the antibacterial activities were tested against seven key strains of antibiotic-resistant bacteria.
However, when the DLLME-SFO method was validated under optimal conditions, the range of values of limit of detection, limit of quantification, dynamic linearity range, determination of coefficient, enrichment factor and extraction recovery were (0.025 0.377 ng mL−1), (0.083 1.256 ng mL−1), (0.100 1.000 ng mL−1), (R2 = 0.990 0.999), (35 49), (69 98%) respectively.
Random trials were conducted to experimentally validate the evaluated optimal conditions.
Initial fermentation medium (FM1), single-factor optimum medium (FM2), and BBD optimum medium (FM3) were investigated and evaluated to validate the predicted optimal conditions (Table 4).
The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (±0.3%) was obtained.
Four extractions were performed in parallel at the optimal conditions to validate the model.
A repeat fermentation for α-L-arabinofuranosidase production was carried out under optimal conditions to validate the parameters predicted by the model.
Finally, the optimal conditions were experimentally validated, and AMVL production with optimized culture media was compared with the initial conditions.
Simultaneous optimization of the responses was conducted and validated by performing experiments at the optimal conditions.
An experiment was performed under the predicted optimal conditions in order to validate the optimized medium.
