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The qPCR results showed that 10 of the 12 selected DEGs were successfully validated (consistent tendency) while 2 DEGs were not (data not shown).
The simulated model was validated by the consistent results between the calculated and experimental data.
The simulation and experiment give the consistent results, which validate our theoretical inference.
However, it is apparent from Figure 5 that the 10% cutoff, used in all the studies except those of Kaiser et al (1996) (50% cutoff) and Lee et al (2008) (both extent and intensity of staining), has not been validated, as it does not give consistent results and the 25% cutoff for β1-integrin has only been reported in a single cohort.
In addition, the methodology was further validated for assaying spiked seafood samples, and consistent results between the electrochemical immunoassay and the referenced enzyme immunoassay were obtained.
Through benchmark with different grid discretization approaches, the EDFN model provides very consistent results and its accuracy is validated.
Furthermore, we have used two established and validated measures for both insulin sensitivity and β-cell function (13– 17) with consistent results observed in each case.
The strength of our study is mainly the use of a validated ISAAC questionnaire, which has been used in many studies globally, with consistent results.
Moreover, we have used two established and validated measures for both insulin sensitivity and β-cell function, with generally consistent results observed in each case (12– 12).
Consistent results have been obtained with those similar cases from literature, and the robustness and efficiency of the model is validated.
The most consistent results involved education.
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