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Although more than 40 microsatellite-based genome wide linkage scans have been performed and single candidate genes have been detected, none of the results have been validated unequivocally [ 82].
Thirty-eight percent of sites in a validation sample of 1234 sites was unequivocally validated, 23% was equivocally validated, and the remainder was predicted wrongly by the method.
Further, TEM images of breast tumor (HBL-100) cells treated with GSH-GAuNPs and LA-GAuNPs unequivocally validated our hypothesis.
This is the scene in which you completely and unequivocally validate the headphone method and then never ever refer to it again.
However, this hypothesis has not yet been unequivocally validated [ 8, 10].
The identities of these four metabolites were unequivocally validated by comparison to authentic enzymatically synthesized standards.
The qPCR experiments did not unequivocally validate or refute the presence of a duplication in this region (Supplementary Figure 2c).
However, it is difficult to unequivocally validate this possibility without specific technology that can efficiently separate different alleles in cells.
A number of alternative Raf-1 substrates have been described, but none has been unequivocally validated yet.
However, we still lack mouse genetic studies to unequivocally validate its previously assigned putative tumor suppressor function in human lung adenocarcinomas.
Importantly, we have unequivocally validated a biologically relevant role for DCI in the HCV life cycle using a combination of gene silencing and pharmacologic inhibition approaches [ 2, 5, 7].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com