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Cloning from small RNA libraries also validates the expression of microRNAs.
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To further validate the expression of differentially expressed miRNAs from high-throughput sequencing, RT-qPCR was performed on five novel candidate miRNAs and seven randomly selected known miRNAs.
Quantitative RT-PCR was used to validate the expression of select differentially expressed miRNAs bta-miR-199a-3p bta-miR-199a-3p bta-miR-199a-3p bta-miR-199a-3p
We validated the expression of five novel miRNAs and found three to be differentially expressed.
Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress.
In this study, we designed RT-PCR primers using conserved 5′ regions and the mouse 3′ domain to validate the expression of Vamp1.
RT-qPCR was used to validate the expression of 11 selected genes from the microarray analysis.
Our study further validated the expression of these two proteins using the Western blot technique.
We chose to validate the expression of several miRNAs during senescence with quantitative real-time PCR.
We chose to further validate the expression of gadd45α and mmp8.
We validated the expression of eight genes with varying degrees of evidence for involvement in ovarian cancer.
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